High-resolution mass spectrometry and molecular weight determination for peptides and small proteins. Confirm identity, detect modifications, and characterize complex mixtures with ESI-MS and MALDI-TOF.
Peptide molecular analysis uses mass spectrometry to determine the exact molecular weight of a peptide and confirm its primary sequence. At Cert Biohealth Testing Labs, we use ESI-MS and LC-MS/MS to verify identity, detect chemical modifications (oxidation, deamidation, acetylation), characterize impurities, and provide full spectral data. Standard turnaround is 9–11 business days from sample receipt.
Our analytical chemists use state-of-the-art mass spectrometry platforms to characterize peptides at the molecular level, providing data suitable for research publications and regulatory submissions.
Electrospray ionization mass spectrometry to confirm peptide molecular weight and primary sequence identity with high accuracy.
Matrix-assisted laser desorption ionization for rapid molecular weight screening of peptides, proteins, and complex mixtures.
Tandem mass spectrometry coupled with liquid chromatography for comprehensive characterization of peptide sequence and modifications.
Identification of post-synthetic modifications including oxidation, deamidation, acetylation, and disulfide bond formation.
Precise molecular weight measurement with sub-ppm accuracy for quality control and regulatory documentation.
De novo sequencing and sequence tag identification to verify peptide primary structure against theoretical mass.
ESI-MS (electrospray ionization) is best for quantitative work and LC-coupled analysis, producing multiple charge states that allow accurate molecular weight calculation. MALDI-TOF (matrix-assisted laser desorption) is faster for single-point MW screening but less quantitative. We recommend ESI-MS for regulatory-grade COA work and MALDI-TOF for rapid screening of large peptide libraries.
Yes. LC-MS/MS analysis can detect common modifications including oxidation (+16 Da), deamidation (+1 Da), acetylation (+42 Da), disulfide bonds, TFA adducts, and many others. We compare the observed spectrum against the theoretical mass of the target sequence and flag all discrepancies.
Typically 0.5–2 mg of lyophilized peptide. For difficult or very large peptides, we may request up to 5 mg. Contact us at info@certbiohealth.ca to discuss your specific sequence before shipping.
Yes. Raw instrument files (.raw, .d, mzML) are available upon request at no additional cost. These are commonly needed for research publications and grant applications.
Yes. We have experience with cyclic peptides, lipopeptides, PEGylated peptides, and peptides bearing non-natural amino acids. Contact us with your sequence and modification details for a custom quote.
Precise molecular weight determination and sequence verification using high-resolution mass spectrometry. Results in 9–11 business days.
+1 (416) 000-0000 | info@certbiohealth.ca